Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization

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Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization

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Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization

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Título: Tau protein binds to pericentromeric DNA: a putative role for nuclear tau in nucleolar organization
Autor: Sjoberg, Marcela; Shestakova, Elena; Mansuroglu, Zeyni; Maccioni Baraona, Ricardo Benjamín; Bonnefoy, Eliette
Resumen: The microtubule-associated tau protein participates in the organization and integrity of the neuronal cytoskeleton. A nuclear form of tau has been described in neuronal and non-neuronal cells, which displays a nucleolar localization during interphase but is associated with nucleolar-organizing regions in mitotic cells. In the present study, based on immunofluorescence, immuno-FISH and confocal microscopy, we show that nuclear tau is mainly present at the internal periphery of nucleoli, partially colocalizing with the nucleolar protein nucleolin and human AT-rich alpha-satellite DNA sequences organized as constitutive heterochromatin. By using gel retardation, we demonstrate that tau not only colocalizes with, but also specifically binds to, AT-rich alpha-satellite DNA sequences apparently through the recognition of AT- rich DNA stretches. Here we propose a functional role for nuclear tau in relation to the nucleolar organization and/or heterochromatinization of a portion of RNA genes. Since nuclear tau has also been found in neurons from patients with Alzheimer's disease (AD), aberrant nuclear tau could affect the nucleolar organization during the course of AD. We discuss nucleolar tau associated with AT-rich alpha-satellite DNA sequences as a potential molecular link between trisomy 21 and AD.
URI: http://www.captura.uchile.cl/handle/2250/6251
Fecha: 2006-05-15
Cita del item: JOURNAL OF CELL SCIENCE Volume: 119 Issue: 10 Pages: 2025-2034 Published: MAY 15 2006


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