How changes in the sequence of the peptide CLPFFD-NH2 can modify the conjugation and stability of gold nanoparticles and their affinity for beta-amyloid fibrils

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How changes in the sequence of the peptide CLPFFD-NH2 can modify the conjugation and stability of gold nanoparticles and their affinity for beta-amyloid fibrils

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How changes in the sequence of the peptide CLPFFD-NH2 can modify the conjugation and stability of gold nanoparticles and their affinity for beta-amyloid fibrils

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Título: How changes in the sequence of the peptide CLPFFD-NH2 can modify the conjugation and stability of gold nanoparticles and their affinity for beta-amyloid fibrils
Autor: Olmedo Alegría, Ivonne Odette; Araya, Eyleen; Sanz, F.; Medina Consuegra, Elías Daniel; Arbiol, Jordi; Toledo, P.; Álvarez Lueje, Alejandro Francisco; Giralt, Ernest; Kogan, Marcelo J.
Resumen: In a previous work, we studied the interaction of P-amyloid fibrils (A beta) with gold nanoparticles (AuNP) conjugated with the peptide CLPFFD-NH2. Here, we studied the effect of changing the residue sequence of the peptide CLPFFD-NH2 on the efficiency of conjugation to AuNP, the stability of the conjugates, and the affinity of the conjugates to the A beta fibrils. We conjugated the AuNP with CLPFFD-NH2 isomeric peptides (CDLPFF-NH2 and CLPDFF-NH2) and characterized the resulting conjugates with different techniques including UV-Vis, TEM, EELS, XPS, analysis of amino acids, agarose gel clectrophoresis, and CD. In addition, we determined the proportion of AuNP bonded to the A beta fibrils by ICP-MS. AuNP-CLPFFD-NH2 was the most stable of the conjugates and presented more affinity for A beta fibrils with respect to the other conjugates and bare AuNP. These findings help to better understand the way peptide sequences affect conjugation and stability of AuNP and their interaction with A beta fibrils. The peptide sequence, the steric effects, and the charge and disposition of hydrophilic and hydrophobic residues are crucial parameters when considering the design of AuNP peptide conjugates for biomedical applications.
URI: http://www.captura.uchile.cl/handle/2250/5175
Fecha: 2008-06
Cita del item: BIOCONJUGATE CHEMISTRY 19(6):1154-1163


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