Polarographic reduction of megazol and derivatives, and its polarographic, UV spectrophotometric, and HPLC determination

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Polarographic reduction of megazol and derivatives, and its polarographic, UV spectrophotometric, and HPLC determination

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Polarographic reduction of megazol and derivatives, and its polarographic, UV spectrophotometric, and HPLC determination

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Título: Polarographic reduction of megazol and derivatives, and its polarographic, UV spectrophotometric, and HPLC determination
Autor: Bollo Dragnic, Soledad; Núñez Vergara, Luis J.; Bontá, M.; Chauviere, G.; Perie, J.; Squella Serrano, Juan Arturo
Resumen: Megazol (2-amino-5-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazol, CAS 19622-55-0), a highly active compound used against several of Trypanosoma cruzi, the causative agent of Chagas disease, is electrochemically studied to propose a new electroanalytical strains, of Try alternative for its quantitative determination. Megazol is shown to be polarographically reducible in protic and mixed media. The polarogram of megazol shows two signals, the main signal is due to the reduction of the nitroimidazole moiety, and the second one is due to the reduction of the thiadiazole moiety in the molecule. We have synthesized several related molecules to megazol in order to study the influence of structural changes in the reducibility of the nitro group. Also we have compared the polarographic behavior of megazol with the currently used antichagasic drug, benznidazole. Based on the linear relation between the peak current and the megazol concentrations a differential pulse polarographic method was developed. The linearity was maintained between 6 x 10(-6) and 1 x 10(-4) M with a quantitation and detection limits of 6.7 x 10(-6) and 3.2 x 10(-6) M, respectively. Precision and accuracy of the developed method was checked by recovery study. For comparative purposes spectrophotometric and HPLCuv methodologies were developed. From the pH dependence of the absorbance band at 276 nm. an apparrent pK(a) of 8.5 was determined for megazol.
URI: http://www.captura.uchile.cl/handle/2250/5141
Fecha: 2001-07
Cita del item: ELECTROANALYSIS 13(11):936-943


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