Marlin-1 and conventional kinesin link GABA(B) receptors to the cytoskeleton and regulate receptor transport

Manakin: DSpace XMLUI Project v2

Marlin-1 and conventional kinesin link GABA(B) receptors to the cytoskeleton and regulate receptor transport

Cómo citar
Cómo citar

Marlin-1 and conventional kinesin link GABA(B) receptors to the cytoskeleton and regulate receptor transport

.
Copiar
Título: Marlin-1 and conventional kinesin link GABA(B) receptors to the cytoskeleton and regulate receptor transport
Autor: Vidal, René L.; Ramírez, Omar A.; Sandoval, Lisette; Koenig Robert, Roger; Haertel, Steffen; Couve, Andrés
Resumen: The cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABA(B) receptors (GABA(B)Rs) to the cytoskeleton have not been described. We recently identified Marlin-1, a protein that interacts with GABA(B)R1. Here, we explore the association of GABA(B)Rs and Marlin-1 to the cytoskeleton using a combination of biochemistry, microscopy and live cell imaging. Our results indicate that Marlin-1 is associated to microtubules and the molecular motor kinesin-I. We demonstrate that a fraction of Marlin-1 is mobile in dendrites of cultured hippocampal neurons and that mobility is microtubule-dependent. We also show that GABA(B)Rs interact robustly with kinesin-I and that intracellular membranes containing GABA(B)Rs are sensitive to treatments that disrupt a protein complex containing Marlin-1, kinesin-I and tubulin. Finally, we report that a kinesin-I mutant severely impairs receptor transport. We conclude that Marlin-1 and kinesin-1 link GABA(B)Rs to the tubulin cytoskeleton in neurons. (c) 2007 Elsevier Inc. All rights reserved.
Descripción: Publicación ISIEmail : andres@neuro.med.uchile.cl
URI: http://www.captura.uchile.cl/handle/2250/5040
Fecha: 2007
Cita del item: MOLECULAR AND CELLULAR NEUROSCIENCE Vol. 35 JUL 2007 3 501-512


Archivos vinculados

Formato: Nombre: Tamaño: Descripción: Ver
Fichero de texto
file_5294.txt
0bytes
Abrir / Descargar

Este ítem aparece en la(s) siguiente(s) colección(ones)