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Title:
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4-DIMETHYLAMINOPHENETHYLAMINE, A SENSITIVE, SPECIFIC, ELECTROCHEMICALLY DETECTABLE MONOAMINE OXIDASE-B SUBSTRATE |
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Author:
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Reyes-Parada, Miguel; Scorza, Ma. Cecilia; Silveira, Rodolfo; Dajas, Federico; Cassels, Bruce K.
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Abstract:
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4-Dimethylaminophenethylamine (DMAPEA) was characterized
as an MAO substrate. This compound was unaffected by
MAO-A, while its oxidation by MAO-B was linear as a
function of both time and enzyme concentration, with Km
= 5.8 11M and Vmax = 21.2 pmol/min/mg protein, using a
crude rat brain mitochondrial suspension as source of MAO.
Both DMAPEA and its oxidation product,
4-dimethylaminophenylacetic acid (DMAPAA) , can be detected
electrochemically at 0.85 V. The high MAO-B affinity and
selectivity of DMAPEA, together with its low oxidation
potential, make this molecule a unique tool to determine
MAO-B activity in a wide variety of tissue preparations
using HPLC-ED. |
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URI:
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http://www.captura.uchile.cl/handle/2250/16955
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Date:
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1994-04-04 |
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dc.identifier.citation:
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Lire Sciences, Vol. 54, Nª 25, pp. 1955-1963, 1994 |