ATP-diphosphophydrolase activin in rat heart tissue

Cómo citar
Cómo citar

ATP-diphosphophydrolase activin in rat heart tissue

.
Copiar
Título: ATP-diphosphophydrolase activin in rat heart tissue
Autor: Espinosa, Victoria; Galleguillos, M.; Mancilla, Marta; Garrido, J.; Kettlun, Ana María; Collados, L. E.; Chayet, L.; García, L.; Traverso Cori, A.; Valenzuela, M. A.
Resumen: Extracellular nucleotides interact with specific receptors on the cell surface and are locally metabolized by ecto-nucleotidases. Biochemical characterization of the ATPase and ADPase activities detected in rat heart sarcolemma, under conditions where mitochondrial ATPase and adenylate kinase were blocked, suppor:s our proposal that both activities correspond to a single enzyme, known as ATP-diphosphohydrolase or apyrase. The physiological function of this enzyme could be dephosphorylation of the nucleotides present in the interstitial heart compartment acting together with 5'-nucleotidase. Both hydrolytic activities have similarities in: sarcolemma localization, bivalent metal ion dependence, optimum pH, effect of several amino acid residue modifiers, competitive inhibition of nucleotide analogs, and broad nucleoside di- and triphosphate specificity. The ATPase activity could not be separated from the ADPase either through isoelectrofocusing or electrophoresis under acid conditions.
Descripción: Artículo de publicación ISI
URI: http://www.captura.uchile.cl/handle/2250/13879
Fecha: 1996-08
Cita del item: BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 39 (5): 905-915


Archivos vinculados

Formato: Nombre: Tamaño: Descripción: Ver
PDF
ESPINOSA_1996.pdf
8.555Mb
Abrir / Descargar

Este ítem aparece en la(s) siguiente(s) colección(ones)