Transforming Growth Factor-beta and All-Trans Retinoic Acid Generate Ex Vivo Transgenic Regulatory T Cells With Intestinal Homing Receptors

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Transforming Growth Factor-beta and All-Trans Retinoic Acid Generate Ex Vivo Transgenic Regulatory T Cells With Intestinal Homing Receptors

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Transforming Growth Factor-beta and All-Trans Retinoic Acid Generate Ex Vivo Transgenic Regulatory T Cells With Intestinal Homing Receptors

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Título: Transforming Growth Factor-beta and All-Trans Retinoic Acid Generate Ex Vivo Transgenic Regulatory T Cells With Intestinal Homing Receptors
Autor: Moore, C.; Sauma, Daniela; Morales, J.; Bono, M. R.; Rosemblatt, M.; Fierro, J. A.
Resumen: CD4 CD25 Foxp3 regulatory T cells (Treg) mediate immunologic self-tolerance and suppress immune responses. In the gut, a subset of dendritic cells is specialized to induce Treg in a transforming growth factor- (TGF- )- and retinoic acid (RA)-dependent manner. The aim of this study was to establish if RA synergizing with TGF- induced antigen specific CD4 CD25high Foxp3 Treg portraying gut homing receptors. Splenic CD4 CD25 Foxp3 naïve T cells from DO11.10 mice were cocultured with splenic CD11c dendritic cells from Balb/c mice in the presence of TGF- , RA, and low levels of an antigenic peptide. After 5 days of culture, cells were analyzed for the expression of Foxp3 and the gut homing receptors CCR9 and 4 7. The number of Foxp3 T cells generated with TGF- and RA was at least 3 times higher than in the cultures with TGF- alone and 15 times higher than in controls without exogenous cytokines. Also, supplementation of the cultures with RA induced the expression of the intestinal homing receptors CCR9 and 4 7. Our results showed that coculture of naïve T cells with antigen-presenting cells in the presence of TGF- and RA represents a powerful approach to generate Treg with specific homing receptors.
URI: http://www.captura.uchile.cl/handle/2250/13369
Fecha: 2009-07
Cita del item: TRANSPLANTATION PROCEEDINGS, Volume: 41, Issue: 6, Pages: 2670-2672, 2009


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