Neuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with alpha v beta 3 integrin that activates PKC alpha and RhoA

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Neuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with alpha v beta 3 integrin that activates PKC alpha and RhoA

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Neuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with alpha v beta 3 integrin that activates PKC alpha and RhoA

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Título: Neuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with alpha v beta 3 integrin that activates PKC alpha and RhoA
Autor: Avalos, Ana María; Valdivia, A. D.; Muñoz, Nicolás; Herrera Molina, Rodrigo; Tapia, Julio C.; Lavandero González, Sergio; Chiong Lay, Mario; Burridge, Keith; Schneider, P.; Quest, Andrew F. G.; Leyton, Lisette
Resumen: Clustering of alpha v beta 3 integrin after interaction with the RGD-like integrin-binding sequence present in neuronal Thy-1 triggers formation of focal adhesions and stress fibers in astrocytes via RhoA activation. A putative heparin-binding domain is present in Thy-1, raising the possibility that this membrane protein stimulates astrocyte adhesion via engagement of an integrin and the proteoglycan syndecan-4. Indeed, heparin, heparitinase treatment and mutation of the Thy-1 heparin-binding site each inhibited Thy-1-induced RhoA activation, as well as formation of focal adhesions and stress fibers in DI TNC1 astrocytes. These responses required both syndecan-4 binding and signaling, as evidenced by silencing syndecan-4 expression and by overexpressing a syndecan-4 mutant lacking the intracellular domain, respectively. Furthermore, lack of RhoA activation and astrocyte responses in the presence of a PKC inhibitor or a dominant-negative form of PKC alpha implicated PKC alpha and RhoA
URI: http://www.captura.uchile.cl/handle/2250/10996
Fecha: 2009-10
Cita del item: JOURNAL OF CELL SCIENCE 122 (19): 3462-3471


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