Perinatal Asphyxia Induces Neurogenesis in Hippocampus: an Organotypic Culture Study

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Perinatal Asphyxia Induces Neurogenesis in Hippocampus: an Organotypic Culture Study

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Perinatal Asphyxia Induces Neurogenesis in Hippocampus: an Organotypic Culture Study

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Título: Perinatal Asphyxia Induces Neurogenesis in Hippocampus: an Organotypic Culture Study
Autor: Morales, P.; Huaiquín, P.; Bustamante, D.; Fiedler, Jenny; Herrera Marschitz, Mario
Resumen: There is clinical and experimental evidence indicating that neurocircuitries of the hippocampus are vulnerable to hypoxia/ischemia occurring at birth, inducing, upon re-oxygenation/re-circulation, delayed neuronal death, but also compensatory mechanisms, including neurogenesis. In the present report, perinatal asphyxia was induced by immersing foetuses-containing uterine horns removed from ready-to-deliver rats into a water bath at 37°C for 20 min. Some pups were delivered immediately after the hysterectomy to be used as non-asphyxiated caesarean-delivered controls. The pups were sacrificed after seven days for preparing organotypic hippocampal cultures. The cultures were grown on a coverslip in a medium-containing culture tube inserted in a hole of a roller device standing on the internal area of a cell incubator at 35°C, 10% CO2. At days in vitro (DIV) 25-27, cultures were fixed for assaying cell proliferation and neuronal phenotype with antibodies against 5-bromo-2'deoxyuridine (BrdU) and microtubule associated protein- 2 (MAP-2), respectively. Confocal microscopy revealed that there was a 2-fold increase of BrdUpositive, but a 40% decrease of MAP-2-positive cells/mm3 in cultures from asphyxia-exposed, compared to that from control animals. Approximately 30% of BrdU-positive cells were also positive for MAP-2 (approximately 4800 cells), mainly seen in the dentate gyrus of the hippocampus, demonstrating a 3-fold increase of postnatal neurogenesis, when the total amount of double-labelled cells seen in cultures from asphyxia-exposed animals is compared to that from control animals.
URI: http://www.captura.uchile.cl/handle/2250/10326
Fecha: 2007
Cita del item: Neurotoxicity Research, 2007, VOL. 12(1). pp. 1-4.


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